3PKS
Urate oxidase under 1.5 MPa / 15 bars pressure of nitrous oxide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.954 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.219, 96.255, 105.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 1.750 |
R-factor | 0.17023 |
Rwork | 0.169 |
R-free | 0.19424 |
Structure solution method | RIGID BODY |
Starting model (for MR) | 2iba |
RMSD bond length | 0.013 |
RMSD bond angle | 1.441 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 40577 | |
<I/σ(I)> | 33.8 | 4.5 |
Completeness [%] | 97.8 | 93.5 |
Redundancy | 4.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 8.5 | 298 | 10-15 mg/ml urate oxidase, 2mg/ml 8-azaxanthine, 50 mM Tris/HCl pH 8.5, 5-8 % PEG 8000, 0-0.05 M NaCl, BATCH, temperature 298K |