3PKH
Urate oxidase under 1.5 MPa / 15 bars pressure of xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-05-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.346, 96.337, 105.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.490 - 1.710 |
| R-factor | 0.16825 |
| Rwork | 0.167 |
| R-free | 0.19143 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.710 |
| Number of reflections | 44320 | |
| <I/σ(I)> | 32.7 | 10.3 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 8.5 | 298 | 10-15 mg/ml Urate oxidase, 2 mg/ml 8-azaxanthine, 50 mM Tris/HCl pH 8.5, 5-8 % PEG 8000, 0-0.05 M NaCl, BATCH, temperature 298K |
