3PJZ

Crystal Structure of the Potassium Transporter TrkH from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	91.489, 97.406, 195.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.995 - 3.506
R-factor	0.2518
Rwork	0.249
R-free	0.29860
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.208
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Rmerge	0.119	0.091	0.249
Number of reflections	20487
<I/σ(I)>	8.9
Completeness [%]	89.5	95.9	71.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.3	298	35% PEG400, 200 mM calcium acetate, 100 mM sodium acetate, pH 5.3, microbatch, temperature 298K
1	MICROBATCH	5.3	298	35% PEG400, 200 mM calcium acetate, 100 mM sodium acetate, pH 5.3, microbatch, temperature 298K