3PJS

Mechanism of Activation Gating in the Full-Length KcsA K+ Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-30
Detector	ADSC QUANTUM 315
Spacegroup name	I 2 2 2
Unit cell lengths	118.266, 176.716, 340.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.800
Rwork	0.281
R-free	0.33220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.290
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.800	3.800
Number of reflections	29324
Completeness [%]	82.4	93.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M Na/K phosphate, 0.1M Bis-Tris propane pH 7.5, 10% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K