3PJR
HELICASE SUBSTRATE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 105.120, 105.120, 380.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.300 |
| R-factor | 0.236 * |
| Rwork | 0.236 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.026 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 3.300 |
| Rmerge | 0.060 |
| Number of reflections | 17365 |
| Completeness [%] | 92.0 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.175 (mM) | |
| 10 | 1 | reservoir | 50 (mM) | ||
| 11 | 1 | reservoir | PEG4000 | 12-20 (%) | |
| 2 | 1 | drop | glucose | 4.8 (%) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | ADPNP | 2.5 (mM) | |
| 5 | 1 | drop | dithiothreitol | 2 (mM) | |
| 6 | 1 | drop | Tris | 26.5 (mM) | |
| 7 | 1 | drop | EDTA | 1 (mM) | |
| 8 | 1 | drop | 106 (mM) | ||
| 9 | 1 | reservoir | Tris | 100 (mM) |
