3PJP
A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain(CTD)
Replaces: 3OR8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-03-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91881 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.240, 57.020, 87.970 |
| Unit cell angles | 90.00, 126.14, 90.00 |
Refinement procedure
| Resolution | 35.040 - 1.600 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.043 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 56066 |
| <I/σ(I)> | 13.9 |
| Redundancy | 2.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22-24% PEG 8000, 50MM MES, 0.2M SODIUM ACETATE, PH 7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
