3PJL

The crystal structure of Tp34 bound to Co (II) ion at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	34.406, 65.984, 152.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.878 - 1.700
R-factor	0.1721
Rwork	0.171
R-free	0.20100
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.425
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	39218
<I/σ(I)>		2.2
Completeness [%]	99.8	99.6
Redundancy		4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bicine, 2.4 M Ammonium Sulfate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K