3PJJ
Synthetic Dimer of Human Carbonic Anhydrase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.705, 51.132, 80.935 |
| Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.22204 |
| Rwork | 0.219 |
| R-free | 0.28261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.922 |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 20672 | |
| Completeness [%] | 78.8 | 78.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.4 M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
