3PJA
Crystal structure of human C3PO complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.250, 95.830, 232.885 |
| Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
| Resolution | 29.916 - 3.000 |
| R-factor | 0.2083 |
| Rwork | 0.206 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.823 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_555)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.093 | 0.683 |
| Number of reflections | 73372 | |
| <I/σ(I)> | 14.96 | 1.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 12% PEG 3350, 75mM potassium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
