3PIW

Zebrafish interferon 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	77
Detector technology	CCD
Collection date	2008-01-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8742,1.5
Spacegroup name	P 61
Unit cell lengths	84.215, 84.215, 45.209
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.813 - 1.492
R-factor	0.1436
Rwork	0.141
R-free	0.18740
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.838
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.932	1.540
High resolution limit [Å]	1.490	1.492
Number of reflections	29444
Completeness [%]	98.4	87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.2 M MgCl2, 22% PEG3350, 0.1 M HEPES pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.2 M MgCl2, 22% PEG3350, 0.1 M HEPES pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K