3PIT
Crystal structure of M-RasD41E in complex with GppNHp (type 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.111, 89.646, 41.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.970 - 1.550 |
| R-factor | 0.18617 |
| Rwork | 0.185 |
| R-free | 0.21271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.063 | 0.283 |
| Number of reflections | 25878 | |
| <I/σ(I)> | 7.4 | 2.7 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 12.9 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Tris-HCl, 0.2M magnesium chloride, 25% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
