3PIR
Crystal structure of M-RasD41E in complex with GppNHp (type 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 109.421, 109.421, 68.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.090 - 2.750 |
| R-factor | 0.21386 |
| Rwork | 0.210 |
| R-free | 0.28682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1s |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.501 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.100 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.085 | 0.293 |
| Number of reflections | 6653 | |
| <I/σ(I)> | 66.5 | 19.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20.6 | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.2M magnesium chloride, 20% PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
