3PIN
Crystal structure of Mxr1 from Saccharomyces cerevisiae in complex with Trx2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 122.266, 122.266, 122.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.23661 |
| Rwork | 0.234 |
| R-free | 0.27882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pil 2fa4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 8320 | |
| Completeness [%] | 98.0 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 289 | 0.2M trimethylamine N-oxide, 0.1M Tris, 20% polyethylene glycol 2000 monomethyl ether, pH 8.5, VAPOR DIFFUSION, temperature 289K |
