3PIK

Outer membrane protein CusC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	H 3 2
Unit cell lengths	89.030, 89.030, 473.037
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.923 - 2.300
R-factor	0.1997
Rwork	0.197
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Pseudomonas aeruginosa OprM
RMSD bond length	0.009
RMSD bond angle	1.024
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	35080
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	35% PEG200, 0.2 M Ammonium sulphate, 50 mM sodium acetate pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 295K