3PIJ
beta-fructofuranosidase from Bifidobacterium longum - complex with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 86.765, 86.765, 223.868 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.400 - 1.800 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.20800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3pig |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.727 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.5.0091) |
| Refinement software | REFMAC (5.5.0091) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.400 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.097 | 0.291 |
| Number of reflections | 84167 | |
| <I/σ(I)> | 13.8 | 1.95 |
| Completeness [%] | 92.1 | 56 |
| Redundancy | 5.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.4M ammonium chloride, 18% PEG 3350, 0.1M MES pH 7.0, protein concentration 11 mg/ml , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
