3PID
The apo-form UDP-glucose 6-dehydrogenase with a C-terminal six-histidine tag
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.970, 63.722, 79.096 |
Unit cell angles | 90.00, 114.70, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.400 |
R-factor | 0.1692 |
Rwork | 0.168 |
R-free | 0.18780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dli |
RMSD bond length | 0.020 |
RMSD bond angle | 1.814 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.350 | 0.403 |
Number of reflections | 94272 | |
<I/σ(I)> | 49.7 | 3 |
Completeness [%] | 98.2 | 86.1 |
Redundancy | 7.2 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1M Tris-HCl buffer (pH 8.0), 0.8M sodium formate (Fluka), 14 % (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |