3PI6
Crystal structure of the CFTR inhibitory factor Cif with the H177Y mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.394, 84.056, 89.325 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 43.930 - 1.500 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kd2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 195124 | |
| <I/σ(I)> | 25.7 | 5.9 |
| Completeness [%] | 99.8 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 16% PEG 8000, 0.125M CALCIUM CHLORIDE, 0.1M SODIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
