3PHS
Crystal Structure of GBS52, the minor pilin in gram-positive pathogen Streptococcus agalactiae
Replaces: 2PZ4Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.481, 52.222, 61.006 |
| Unit cell angles | 90.00, 101.79, 90.00 |
Refinement procedure
| Resolution | 26.810 - 1.800 |
| R-factor | 0.20079 |
| Rwork | 0.199 |
| R-free | 0.23042 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.370 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.810 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.157 |
| Number of reflections | 23655 | |
| <I/σ(I)> | 14.3 | 4.6 |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 3.01 | 2.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% PEG1500, 0.1M HEPES buffer, 0.2M Ammonium Citrate, 0.01M CaCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
