3PHM

REDUCED (CU+) PEPTIDYLGLYCINE ALPHA-HYDROXYLATING MONOOXYGENASE (PHM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	69.519, 68.770, 82.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.2 *
Rwork	0.200
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1phm
RMSD bond length	0.015
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.065 *
Total number of observations	141184 *
Number of reflections	23704
<I/σ(I)>	14.8	3.3
Completeness [%]	99.9	99.6
Redundancy	6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	293 *	pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		1.8 (mM)
3	1	reservoir	sodium cacodylate	100 (mM)	pH5.5
4	1	reservoir		3.08 (mM)