3PHF
Crystal Structure of the Epstein-Barr virus gH and gL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 151.510, 244.909, 287.950 |
| Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
| Resolution | 28.868 - 3.580 |
| Rwork | 0.284 |
| R-free | 0.31280 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.884 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.868 | 28.868 | 3.690 |
| High resolution limit [Å] | 3.500 | 11.070 | 3.500 |
| Rmerge | 0.136 | ||
| Number of reflections | 261791 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.3 | 94.4 | 97.4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 24% PEG 1000, 0.1M Sodium Citrate, 0.2M NDSB256-4T , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
