3PH0

Crystal structure of the heteromolecular chaperone, AscE-AscG, from the type III secretion system in Aeromonas hydrophila

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792, 0.9794, 0.9640, 1.542
Spacegroup name	P 21 21 2
Unit cell lengths	43.159, 71.937, 86.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.239
R-free	0.29230
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400	2.400
Number of reflections	16865
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10mM Tris(pH 7.4), 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K