3PGZ

Crystal structure of a single strand binding protein (SSB) from bartonella henselae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-18
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	93.760, 93.760, 63.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.880 - 2.100
R-factor	0.212
Rwork	0.210
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lgj
RMSD bond length	0.016
RMSD bond angle	1.596
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.061	0.527
Number of reflections	18898
<I/σ(I)>	22.67	3.2
Completeness [%]	98.7	96
Redundancy	6.7	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	290	25 % PEG 3350, 200 MM NACL, 100 MM TRIS HCL, PROTEIN AT 4.5 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K, pH 9.0