3PGA

STRUCTURAL CHARACTERIZATION OF PSEUDOMONAS 7A GLUTAMINASE-ASPARAGINASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	118.260, 130.750, 85.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.165
Rwork	0.165
RMSD bond length	0.019
RMSD bond angle	0.059 *
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.073 *
Total number of observations	212153 *
Number of reflections	81878
Completeness [%]	86.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30-35 (mg/ml)
2	1	drop	phosphate	10 (mM)
3	1	drop	MPD	30-33 (%(v/v))
4	1	reservoir	MPD	35-38 (%(v/v))