3PG2
The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-16 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.872, 60.273, 80.370 |
| Unit cell angles | 90.00, 101.58, 90.00 |
Refinement procedure
| Resolution | 37.150 - 1.800 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.248 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.150 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.290 |
| Number of reflections | 60630 | |
| <I/σ(I)> | 9.6 | 3.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 2.81 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 295 | 25 % PEG MME 2000, 0.1 M MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
