3PF0

Crystal structure of an Imelysin-like protein (Psyc_1802) from PSYCHROBACTER ARCTICUM 273-4 at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795,0.9537,0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	60.674, 69.131, 165.051
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.602 - 2.150
R-factor	0.184
Rwork	0.182
R-free	0.21760
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.602	45.602	2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.064	0.030	0.619
Number of reflections	19285	2062	1976
<I/σ(I)>	11.99	28.5	2
Completeness [%]	99.7	98.7	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2M MgCl2, 30.0% PEG-4000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K