3PEW

S. cerevisiae Dbp5 L327V bound to RNA and ADP BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	42.309, 90.810, 105.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.360 - 1.501
R-factor	0.1661
Rwork	0.165
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.503
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.2.0)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.062	0.030	0.328
Number of reflections	64800
<I/σ(I)>	25.1
Completeness [%]	98.8	91.6	97.7
Redundancy	6.9	6.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG 3350, 200 mM MgNO3, 10 mM HEPES pH 7.5, 100 mM NaCl, 1mM DTT, 0.5 mM IP6, 5 mM MgCl2, 1 mM ADP, 3 mM BeCl2, 15 mM NaF, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K