3PEN
Structure of archaeal initiation factor aIF2gamma subunit delta 37-47 from Sulfolobus solfataricus in the GDP-bound form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 192.6 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 187.120, 187.120, 187.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.100 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pmd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.143 | 0.653 |
| Number of reflections | 48144 | |
| <I/σ(I)> | 12.88 | 2.42 |
| Completeness [%] | 93.0 | 83 |
| Redundancy | 3.75 | 3.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 301 | 1.6M Sodium formate, 44mM Sodium cacodylate, GDPNP, GDP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
