Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-04 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.973, 88.045, 53.073 |
| Unit cell angles | 90.00, 103.37, 90.00 |
Refinement procedure
| Resolution | 33.500 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.037 | 0.100 |
| Number of reflections | 29475 | |
| <I/σ(I)> | 23.7 | 9.4 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 1.7 M AmSO4, 100 mM glycine pH 9.0, 0.6 M 3-(1-pyridino)-1-propane sulfonate [NDSB-201] and 21% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
