3PE0
Structure of the central region of the plakin domain of plectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.650, 108.512, 112.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.977 - 2.950 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.26540 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 18051 | |
| <I/σ(I)> | 29.4 | 6.9 |
| Completeness [%] | 93.6 | 85.5 |
| Redundancy | 14.3 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 277 | 0.1 M imidazole, 0.2 M calcium acetate, 9% PEG 8000, 2 mM DTT, pH 7.2, VAPOR DIFFUSION, temperature 277K |
