3PDY

Structure of the third and fourth spectrin repeats of the plakin domain of plectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-05-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	97.420, 119.400, 44.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.983 - 2.218
R-factor	0.2058
Rwork	0.203
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model based on PDB entry 2IAK
RMSD bond length	0.004
RMSD bond angle	0.679
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.218	2.218
Number of reflections	26072
<I/σ(I)>	23.1	4.9
Completeness [%]	98.4	92.1
Redundancy	7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	297	0.1M HEPES, 17% PEG 4000, 8% ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K