3PDV
Structure of the PDlim2 PDZ domain in complex with the C-terminal 6-peptide extension of NS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 81.917, 81.917, 81.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.310 - 2.200 |
| R-factor | 0.20009 |
| Rwork | 0.198 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 4548 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.2M NaCl, 0.1M HEPES, 22.5% PEG3350, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
