3PDT
Crystal Structure of the C-terminal Truncated Alpha-Kinase Domain of Myosin Heavy chain Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9170 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.062, 83.681, 44.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.390 - 1.800 |
| R-factor | 0.20141 |
| Rwork | 0.199 |
| R-free | 0.24259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lkm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 26646 | |
| <I/σ(I)> | 21.8 | 3.9 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 18% PEG 8000, 0.2M sodium phosphate,0.1M Tris.HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
