3PDK
crystal structure of phosphoglucosamine mutase from B. anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2009-05-14 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.064, 86.064, 266.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.030 - 2.700 |
| R-factor | 0.21759 |
| Rwork | 0.214 |
| R-free | 0.28052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f7l |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.865 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.090 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.074 | 0.521 |
| Number of reflections | 32427 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 5.83 | 6.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M sodium acetate, 2.0 M Na/K phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
