Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0079 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 32.877, 37.911, 79.430 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 25.322 - 1.260 |
| R-factor | 0.1141 |
| Rwork | 0.114 |
| R-free | 0.14440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.893 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.310 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.039 | 0.242 |
| Number of reflections | 51641 | |
| <I/σ(I)> | 53.6 | 8.4 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 9.2 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 20% PEG 4000, 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
