3PBX
Strontium bound to the sequence d(CCGGCGCCGG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | OXFORD SAPPHIRE CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.890, 25.001, 37.044 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 14.860 - 1.879 |
| R-factor | 0.2544 |
| Rwork | 0.251 |
| R-free | 0.32701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3goj |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.666 |
| Data reduction software | CrysalisPro (Red) |
| Data scaling software | CrysalisPro (Red) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.866 | 1.927 |
| High resolution limit [Å] | 1.879 | 1.879 |
| Rmerge | 0.045 | |
| Number of reflections | 3706 | |
| Completeness [%] | 78.5 | |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | SrCl2, MPD, NaCacodylate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
