3PAM
Crystal structure of a domain of transmembrane protein of ABC-type oligopeptide transport system from Bartonella henselae str. Houston-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.847, 39.715, 101.353 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 36.950 - 2.310 |
| R-factor | 0.22496 |
| Rwork | 0.223 |
| R-free | 0.25684 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.490 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 31961 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 17 % PEG 4000, 8.5% 2-Propanol, Hepes 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
