3P9N
Rv2966c of M. tuberculosis is a RsmD-like methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.780, 54.780, 100.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.390 - 1.900 |
| R-factor | 0.18038 |
| Rwork | 0.178 |
| R-free | 0.22149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fhp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.527 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14223 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 100mM tris(7.4),3.0M potassium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
