3P9M
Crystal Structure of H2-Kb in complex with a mutant of the chicken ovalbumin epitope OVA-G4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-20 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.979, 85.694, 89.290 |
| Unit cell angles | 90.00, 111.50, 90.00 |
Refinement procedure
| Resolution | 41.540 - 2.000 |
| R-factor | 0.1829 |
| Rwork | 0.180 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vac |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.049 | 0.018 | 0.203 |
| Rmeas | 0.021 | 0.238 | |
| Number of reflections | 60157 | 506 | 7813 |
| <I/σ(I)> | 20.75 | 51.8 | 7 |
| Completeness [%] | 94.6 | 93 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.2M calcium acetate, 15% PEG 8K, 0.1M Na-malonate, pH 6, vapor diffusion, hanging drop, temperature 277K |
