3P9L
Crystal Structure of H2-Kb in complex with the chicken ovalbumin epitope OVA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-13 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.990, 90.410, 89.740 |
| Unit cell angles | 90.00, 111.71, 90.00 |
Refinement procedure
| Resolution | 31.042 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vac |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.048 | 0.021 | 0.253 |
| Rmeas | 0.025 | 0.291 | |
| Number of reflections | 65977 | 514 | 8871 |
| <I/σ(I)> | 19.12 | 42.8 | 5.7 |
| Completeness [%] | 98.1 | 90.7 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M calcium acetate, 15% PEG 8K, 0.1M Na-cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 277K |
