3P9F
Urate oxidase-azaxanthine-azide ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.982 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.730, 95.177, 104.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3l8w |
| RMSD bond length | 0.030 |
| RMSD bond angle | 0.073 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.700 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 | 0.180 |
| Number of reflections | 43286 | |
| <I/σ(I)> | 25 | 4.4 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 291 | 50 MM TRIS BUFFER, 0.3 M SODIUM AZIDE, SATURATED WITH AZAXANTHINE, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
