3P9A

An atomic view of the nonameric small terminase subunit of Bacteriophage P22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	76.480, 100.900, 89.847
Unit cell angles	90.00, 93.73, 90.00

Refinement procedure

Resolution	14.997 - 1.755
R-factor	0.178
Rwork	0.177
R-free	0.21650
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.918
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.850
High resolution limit [Å]	1.750	1.750
Number of reflections	126076
<I/σ(I)>	28.2
Completeness [%]	93.5	62.5
Redundancy		3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 0.2 M Potassium Thiocyanate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 0.2 M Potassium Thiocyanate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K