3P96

Crystal structure of Phosphoserine phosphatase SerB from Mycobacterium avium, native form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9774
Spacegroup name	I 2 2 2
Unit cell lengths	65.530, 109.190, 134.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.970 - 2.050
R-factor	0.231
Rwork	0.229
R-free	0.28100
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.540
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.970	36.970	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.062	0.018	0.554
Number of reflections	29584	373	1915
<I/σ(I)>	16.41	45.4	3.7
Completeness [%]	96.5	93.2	85.8
Redundancy	5.7		4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	290	0.2 M MgCl2, 0.1 M MES, 20% PEG6000, protein at 27.45mg/ml, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K