3P91

Crystal structure of Proliferating Cellular Nuclear Antigen from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	141.090, 141.090, 49.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.663 - 2.400
R-factor	0.1507
Rwork	0.148
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vym
RMSD bond length	0.007
RMSD bond angle	1.027
Data reduction software	DENZO
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.664	26.663	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge	0.089	0.059	0.157
Total number of observations		1667	8075
Number of reflections	14275
<I/σ(I)>	10.7	8.7	4.6
Completeness [%]	99.9	97.2	100
Redundancy	3.9	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294.15	1.6 M Sodium citrate, pH 6.5, vapor diffusion, hanging drop, temperature 294.15K