3P90
Crystal Structure Analysis of H207F Mutant of Human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.415, 65.533, 83.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.500 - 2.300 |
| R-factor | 0.2113 |
| Rwork | 0.207 |
| R-free | 0.30420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o3t |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.570 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 83.270 | 2.060 | |
| High resolution limit [Å] | 1.989 | 4.285 | 1.989 |
| <I/σ(I)> | 15.13 | 2.65 | |
| Completeness [%] | 99.3 | ||
| Redundancy | 9.2 | 8.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 293 | 0.1 M Bis-Tris, 30% (v/v) PEG 550 monomethyl ether, 0.05 M calcium chloride, 5 mM DTT, pH 6.5, hanging drop, temperature 293K |
