3P8W
Crystal Structure Analysis of R29M/E81M double mutant of human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.386, 64.610, 82.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.980 - 2.000 |
| R-factor | 0.2141 |
| Rwork | 0.210 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o3t |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.758 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.980 | 1.847 | |
| High resolution limit [Å] | 1.783 | 3.841 | 1.783 |
| <I/σ(I)> | 12.41 | 3.97 | |
| Completeness [%] | 98.2 | ||
| Redundancy | 7.44 | 4.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 25% (w/v) PEG 2000 monomethyl ether, 0.02% azide, 1 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
