3P8M
Human dynein light chain (DYNLL2) in complex with an in vitro evolved peptide dimerized by leucine zipper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.839, 68.415, 101.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.310 - 2.900 |
| R-factor | 0.25 |
| Rwork | 0.248 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xqq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.119 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.760 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.131 | 0.576 |
| Number of reflections | 8273 | |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 94.4 | 96.6 |
| Redundancy | 3.23 | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 20% PEG8000, 0.2 M MgCl2, 0.1 M TRIS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
