3P8A
Crystal Structure of a hypothetical protein from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 148.180, 49.730, 67.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 1.950 |
| R-factor | 0.2398 |
| Rwork | 0.238 |
| R-free | 0.27030 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 37456 | |
| <I/σ(I)> | 17.4 | 5.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.4 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 10 % PEG5KMME, 5 % Tacsimate, 0.1 M Bis-Tris, 0.01 M MgCl2, pH 5.5, VAPOR DIFFUSION, temperature 293K |
