3P7L
Rat Insulin Degrading Enzyme (Insulysin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-11-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9718 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.610, 70.980, 114.000 |
| Unit cell angles | 90.00, 92.66, 90.00 |
Refinement procedure
| Resolution | 41.966 - 2.078 |
| R-factor | 0.2057 |
| Rwork | 0.202 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.077 | 2.077 |
| Number of reflections | 54422 | |
| Completeness [%] | 97.6 | 87.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 mM sodium citrate pH 6.5, 100 mM ammonium acetate, and 20% PEG 4000, 8 mg/ml protein, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
