3P7K
GP41 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-03 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 41.680, 41.680, 151.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| Rwork | 0.316 |
| R-free | 0.32060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 3773 |
| Completeness [%] | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | equal volume of 10mg/mL peptide and mother liquor: 5-10% ethylene glycol, 10% PEG 4000, 24-28% isopropanol, 0.1 M Na citrate, 1% beta-octylglucoside, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
