3P5X
Actinidin from Actinidia arguta planch (Sarusashi)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-15 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 2.29 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.519, 56.039, 70.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.200 |
| R-factor | 0.1739 |
| Rwork | 0.168 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.077 |
| Number of reflections | 10212 | |
| <I/σ(I)> | 51.3 | 33.8 |
| Completeness [%] | 99.3 | 94.2 |
| Redundancy | 6.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | 30% PEG 400, 0.1M sodium acetate, 30mM Cadmium chloride , pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
